How To Create Real-Time PCR Primers Using Primer-BLAST
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A PCR primer is usually designed so that its length is within aspecific range that is usually 18 - 30 nucleotides.a) Briefly explain why a MINIMUM length is recommended for a PCRprimer.b) Briefly explain why there is a MAXIMUM length recommended fora PCR primer.c) This minimum length of a PCR primer can be slightly decreasedif the source of the target DNA (the area you want to amplify) iscontained within a less complex DNA sample (i.e. a DNA fragment ora plasmid vector) than when it is contained
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