Complementary DNA (cDNA) libraries are standard tools for gene expression studies. In theory, the library contains full-length DNA copies of every mRNA in the starting sample, in abundances ...

Restriction mapping is a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints. This method relies upon the use of proteins ...

Displayed here are 23 pairs of chromosomes -- one copy of the human genome. How is the genome sequenced? Let's do a little sequencing ourselves to find out... Current technology lets us read only 500 ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

RADseq has fuelled studies in ecological, evolutionary and conservation genomics by using next-generation sequencing to uncover hundreds or thousands of polymorphic loci across the genome in a single, ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...