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Nature Protocols - Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions Skip to main content Thank you for visiting nature.com.
This new cell culture media enables transfection at 2.5 x 10 6 cells/mL, compared with traditional protocols that recommend transfection at 1.0 x 10 6 cells/mL.
The maximal number of GFP-expressing cells (approximately 60%) is detected at cell densities between 0.5 x 10 6 –1.0 x 10 6 cells/mL at the time of transfection. Figure 1.
Cell transfection has been a transformative tool for understanding cellular function. Accurately measuring transfection ...
But current methods give sub-optimal transfection efficiencies. In 18 July Nature, Uday Tirlapur and Karsten König at the Friedrich Schiller University, Jena, Germany, show that a femtosecond pulse ...
The CellPore™ Transfection System represents a substantial leap forward for cell engineering researchers, offering a novel method—known as mechanoporation—for cargo delivery into mammalian ...
Polyplus-transfection SA has announced that Kempbio, Inc., a provider of high quality bioservices to the biotechnology and biopharmaceutical industry, has signed a license agreement to use ...
The CellPore™ Transfection System represents a substantial leap forward for cell engineering researchers, offering a novel method—known as mechanoporation—for cargo delivery into mammalian ...
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