It follows a previous report showing that the Werner syndrome protein WRN and its interacting protein WRNIP1 are indispensable for translesion DNA synthesis (TLS) by Y-family DNA polymerases (Pols).

Exonuclease activity of hPMC2. To determine the active site concentration of WT hPMC2, we performed multiple-turnover experiments, that is, several rounds of enzyme catalysis, in which the enzyme ...

Mitochondrial DNA (mtDNA) polymerase γ (POLγ) harbours a 3′–5′ exonuclease proofreading activity. Here we demonstrate that this activity is required for the creation of ligatable ends ...

The Family DNA polymerases include Tth and Tma DNA polymerase alongside Taq and have 5′-3′ exonuclease activity, but normally lack 3′-5′.

Background: DNA polymerases ε (POLE) and δ1 (POLD1) encode enzymes involved in DNA replication and proofreading.Somatic POLE/D1 mutations occur in 1-3% of cancers.Loss-of-function alterations ...

Methods: We are recruiting patients with curatively resected, stage III colon cancer which are dMMR or have a centrally confirmed POLE exonuclease domain mutation. Eligible patients are randomised in ...